{"id":569,"date":"2026-05-25T23:35:33","date_gmt":"2026-05-25T15:35:33","guid":{"rendered":"https:\/\/www.shiweitec.com\/?p=569"},"modified":"2026-05-31T11:49:13","modified_gmt":"2026-05-31T03:49:13","slug":"%e8%81%9a%e8%b0%b7%e6%b0%a8%e9%85%b8%e5%9f%ba%e6%a0%91%e6%9e%9d%e7%8a%b6%e5%a4%9a%e8%82%bd%e7%9a%84%e5%8f%af%e6%8e%a7%e5%90%88%e6%88%90%e4%b8%8e%e6%9e%84%e8%b1%a1%e8%b0%83%e6%8e%a7","status":"publish","type":"post","link":"https:\/\/www.shiweitec.com\/index.php\/2026\/05\/25\/%e8%81%9a%e8%b0%b7%e6%b0%a8%e9%85%b8%e5%9f%ba%e6%a0%91%e6%9e%9d%e7%8a%b6%e5%a4%9a%e8%82%bd%e7%9a%84%e5%8f%af%e6%8e%a7%e5%90%88%e6%88%90%e4%b8%8e%e6%9e%84%e8%b1%a1%e8%b0%83%e6%8e%a7\/","title":{"rendered":"\u805a\u8c37\u6c28\u9178\u57fa\u6811\u679d\u72b6\u591a\u80bd\u7684\u53ef\u63a7\u5408\u6210\u4e0e\u6784\u8c61\u8c03\u63a7"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-custom-color-5-color\"><strong>\u6458\u8981<\/strong><\/mark><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u4ee5\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178\uff08Bz-Glu\uff09\u4e3a\u539f\u6599\uff0c\u7ecf\u4e09\u5149\u6c14\u73af\u5316\u5236\u5907\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178N-\u7fa7\u57fa\u9178\u9150\uff08Glu-NCA\uff09\uff1b\u901a\u8fc7<strong>0\u2103\u5f00\u73af\u805a\u5408<\/strong>\u5408\u6210\u7aef\u57fa\u9ad8\u6d3b\u6027\u7ebf\u6027\u805a\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178\uff08PBG\uff09\uff1b\u5229\u7528HBr\/TFA\u9009\u62e9\u6027\u8131\u4fdd\u62a4\u5f15\u5165\u53ef\u63a7\u7fa7\u57fa\u4f4d\u70b9\uff1b\u91c7\u7528<strong>DIC\/HOBt\u80bd\u5076\u8054\u4f53\u7cfb<\/strong>\uff0c\u7ecf\u201c\u8131\u4fdd\u62a4\u2013\u63a5\u679d\u201d\u5faa\u73af\uff0c\u6784\u5efaG0\uff5eG3\u4ee3<strong>\u5355\u5206\u6563\u6811\u679d\u72b6PBG<\/strong>\u3002\u7ed3\u679c\u8868\u660e\uff1a\u4f4e\u6e29\u805a\u5408\u663e\u8457\u6291\u5236\u94fe\u7aef\u73af\u5316\uff0c\u4f2f\u80fa\u7aef\u57fa\u4fdd\u7559\u7387&gt;98%\uff1bDMSO\u66ff\u4ee3DMF\u53ef\u6d88\u9664\u03b1-\u87ba\u65cb\u4f4d\u963b\uff0cG1\u4ee3\u63a5\u679d\u7387\u63d0\u5347\u81f353%\uff1bG3\u4ee3\u5206\u5b50\u91cf\u8fbe1.1\u00d710\u2076\uff0c\u5206\u5b50\u91cf\u5206\u5e03\u6307\u6570\u22641.06\uff1b\u6eb6\u5242\u53ef\u8bf1\u5bfc<strong>\u03b1-\u87ba\u65cb\u2013\u65e0\u89c4\u7ebf\u56e2\u53ef\u9006\u8f6c\u53d8<\/strong>\uff0cDMF\u4e2d\u6d41\u4f53\u529b\u5b66\u76f4\u5f8424.5 nm\uff0cDMSO\u4e2d34.5 nm\u3002\u8be5\u6811\u679d\u72b6\u591a\u80bd\u517c\u5177\u9ad8\u5355\u5206\u6563\u6027\u3001\u751f\u7269\u76f8\u5bb9\u6027\u4e0e\u6784\u8c61\u53ef\u8c03\u6027\uff0c\u4e3a\u836f\u7269\u9012\u9001\u4e0e\u86cb\u767d\u6a21\u62df\u63d0\u4f9b\u7406\u60f3\u5e73\u53f0\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u5173\u952e\u8bcd<\/strong>\uff1a\u805a\u8c37\u6c28\u9178\uff1b\u6811\u679d\u72b6\u591a\u80bd\uff1b\u5f00\u73af\u805a\u5408\uff1b\u53ef\u63a7\u8131\u4fdd\u62a4\uff1b\u6784\u8c61\u8c03\u63a7\uff1b\u5355\u5206\u6563\u6027<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">1 \u5f15\u8a00<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u805a\u8c37\u6c28\u9178\uff08PGA\uff09\u662f\u4e00\u7c7b\u7531\u8c37\u6c28\u9178\u5355\u4f53\u901a\u8fc7\u9170\u80fa\u952e\u8fde\u63a5\u5f62\u6210\u7684<strong>\u751f\u7269\u53ef\u964d\u89e3\u3001\u4f4e\u514d\u75ab\u539f\u6027<\/strong>\u591a\u80bd\uff0c\u5728\u836f\u7269\u8f7d\u4f53\u3001\u7ec4\u7ec7\u5de5\u7a0b\u3001\u7eb3\u7c73\u9012\u9001\u7cfb\u7edf\u7b49\u9886\u57df\u5177\u6709\u91cd\u8981\u5e94\u7528\u4ef7\u503c\u3002\u6839\u636e\u8fde\u63a5\u65b9\u5f0f\uff0cPGA\u5206\u4e3a\u03b1-\u805a\u8c37\u6c28\u9178\u4e0e\u03b3-\u805a\u8c37\u6c28\u9178\uff1a\u03b3-PGA\u591a\u7531\u5fae\u751f\u7269\u53d1\u9175\u5236\u5907\uff0c\u800c\u03b1-PGA\u53ca\u62d3\u6251\u7ed3\u6784\u53ef\u63a7\u7684\u884d\u751f\u7269\uff08\u68b3\u5f62\u3001\u661f\u5f62\u3001\u6811\u679d\u72b6\uff09\u9700\u901a\u8fc7<strong>\u5316\u5b66\u5408\u6210<\/strong>\u5b9e\u73b0\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u6811\u679d\u72b6\u591a\u80bd\uff08Arborescent Polypeptides\uff09\u517c\u5177\u6811\u679d\u72b6\u805a\u5408\u7269\u7684<strong>\u7cbe\u786e\u652f\u5316\u7ed3\u6784<\/strong>\u4e0e\u591a\u80bd\u7684<strong>\u751f\u7269\u76f8\u5bb9\u6027<\/strong>\uff0c\u53ef\u6a21\u62df\u7403\u72b6\u86cb\u767d\u7684\u7a7a\u95f4\u6784\u8c61\uff0c\u540c\u65f6\u5177\u5907\u5355\u5206\u6563\u6027\u3001\u5185\u90e8\u7a7a\u8154\u4e0e\u53ef\u4fee\u9970\u8868\u9762\uff0c\u662f\u7406\u60f3\u7684\u836f\u7269\u8f7d\u4f53\u4e0e\u86cb\u767d\u6a21\u578b\u3002\u76ee\u524d\u6811\u679d\u72b6\u591a\u80bd\u5408\u6210\u4e3b\u8981\u91c7\u7528\u201c\u4ece\u8868\u9762\u751f\u957f\uff08grafting from\uff09\u201d\u6cd5\uff0c\u4f46\u6613\u5bfc\u81f4\u5206\u5b50\u91cf\u5206\u5e03\u5bbd\uff08Mw\/Mn&gt;1.3\uff09\u3001\u7ed3\u6784\u7f3a\u9677\u591a\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u672c\u6587\u91c7\u7528<strong>\u201c\u63a5\u679d\u5230\u8868\u9762\uff08grafting onto\uff09\u201d\u7b56\u7565<\/strong>\uff0c\u4ee5Glu-NCA\u4e3a\u5355\u4f53\uff0c\u7ecf\u4f4e\u6e29\u5f00\u73af\u805a\u5408\u3001\u53ef\u63a7\u8131\u4fdd\u62a4\u3001\u9010\u6b65\u63a5\u679d\uff0c\u5408\u6210G0\uff5eG3\u4ee3\u6811\u679d\u72b6PBG\u3002\u7cfb\u7edf\u7814\u7a76\u805a\u5408\u6e29\u5ea6\u3001\u8131\u4fdd\u62a4\u5ea6\u3001\u6eb6\u5242\u6548\u5e94\u4e0e\u5076\u8054\u6761\u4ef6\u5bf9\u4ea7\u7269\u7ed3\u6784\u4e0e\u6027\u80fd\u7684\u5f71\u54cd\uff0c\u9610\u660e<strong>\u6eb6\u5242\u8bf1\u5bfc\u6784\u8c61\u8f6c\u53d8\u673a\u5236<\/strong>\uff0c\u4e3a\u9ad8\u6027\u80fd\u751f\u7269\u533b\u7528\u591a\u80bd\u6750\u6599\u5f00\u53d1\u63d0\u4f9b\u65b0\u8def\u5f84\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"544\" src=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1024x544.jpg\" alt=\"\" class=\"wp-image-570\" srcset=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1024x544.jpg 1024w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-300x159.jpg 300w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-768x408.jpg 768w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1536x816.jpg 1536w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-600x319.jpg 600w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image.jpg 1736w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">2 \u5b9e\u9a8c\u90e8\u5206<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">2.1 \u8bd5\u5242\u4e0e\u4eea\u5668<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178\uff08Bachem\uff0c99%\uff09\u3001\u4e09\u5149\u6c14\uff08Aldrich\uff0c98%\uff09\u3001\u6b63\u5df1\u80fa\u3001N,N&#8217;-\u4e8c\u5f02\u4e19\u57fa\u78b3\u4e8c\u4e9a\u80fa\uff08DIC\uff09\u30011-\u7f9f\u57fa\u82ef\u5e76\u4e09\u6c2e\u5511\uff08HOBt\uff09\u3001\u4e09\u6c1f\u4e59\u9178\uff08TFA\uff09\u3001HBr\/\u4e59\u9178\u6eb6\u6db2\uff0833%\uff09\u3001\u65e0\u6c34DMF\u3001\u65e0\u6c34DMSO\u3001\u4e59\u9178\u4e59\u916f\u3001\u6b63\u5df1\u70f7\u3001\u7532\u9187\u3001\u4e59\u919a\u5747\u4e3a\u5206\u6790\u7eaf\u6216\u8d85\u5e72\u6eb6\u5242\uff0c\u76f4\u63a5\u4f7f\u7528\u6216\u7ecfCaH\u2082\u51cf\u538b\u84b8\u998f\u7eaf\u5316\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Bruker 300 MHz\/500 MHz\u6838\u78c1\u5171\u632f\u4eea\u3001Waters SEC-MALLS\u7cfb\u7edf\u3001Brookhaven BI-200 SM\u52a8\u6001\u5149\u6563\u5c04\u4eea\u3001\u771f\u7a7a\u5e72\u71e5\u7bb1\u3001\u4f4e\u6e29\u53cd\u5e94\u6d74\u3001\u6c2e\u6c14\u4fdd\u62a4\u88c5\u7f6e\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.2 \u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178N-\u7fa7\u57fa\u9178\u9150\uff08Glu-NCA\uff09\u7684\u5408\u6210<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u5c0610.0 g\uff0842.0 mmol\uff09Bz-Glu\u60ac\u6d6e\u4e8e300 mL\u65e0\u6c34\u4e59\u9178\u4e59\u916f\uff0c\u6c2e\u6c14\u4fdd\u62a4\u4e0b\u52a0\u70ed\u81f3\u56de\u6d41\uff1b\u7f13\u6162\u52a0\u51654.8 g\uff0816 mmol\uff09\u4e09\u5149\u6c14\uff0c\u56de\u6d413 h\uff1b\u51b0\u6c34\u6d74\u51b7\u53741 h\uff0c\u4f9d\u6b21\u7528\u51b0\u6c34\u30010.5% NaHCO\u2083\u6c34\u6eb6\u6db2\u5feb\u901f\u6d17\u6da4\uff1b\u6709\u673a\u76f8\u7ecf\u65e0\u6c34MgSO\u2084\u5e72\u71e5\u3001\u8fc7\u6ee4\u3001\u51cf\u538b\u6d53\u7f29\u81f3100 mL\uff1b\u52a0\u5165\u7b49\u4f53\u79ef\u51b7\u6b63\u5df1\u70f7\uff0c-20\u2103\u7ed3\u6676\u8fc7\u591c\uff1b\u6c2e\u6c14\u4fdd\u62a4\u4e0b\u8fc7\u6ee4\uff0c\u771f\u7a7a\u5e72\u71e5\u5f97\u767d\u8272\u6676\u4f53Glu-NCA\uff0c\u4ea7\u738792%\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.3 \u7ebf\u6027\u805a\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178\uff08PBG\uff09\u7684\u5f00\u73af\u805a\u5408<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u5c068.00 g\uff0830.4 mmol\uff09Glu-NCA\u6eb6\u4e8e20 mL\u65e0\u6c34DMF\uff0c0\u2103\u6405\u62cc\u6eb6\u89e3\uff1b\u52a0\u5165200 \u03bcL\uff081.52 mmol\uff09\u6b63\u5df1\u80fa\uff08\u5355\u4f53\/\u5f15\u53d1\u5242=20\uff09\uff0c0\u2103\u5bc6\u5c01\u53cd\u5e9472 h\uff1b\u53cd\u5e94\u6db2\u5012\u5165\u7532\u9187\u6c89\u6dc0\uff0c\u8fc7\u6ee4\u3001\u7532\u9187\u591a\u6b21\u6d17\u6da4\uff1b\u771f\u7a7a\u5e72\u71e5\u5f97\u767d\u8272\u7c89\u672b\u7ebf\u6027PBG\uff0c\u4ea7\u738780%\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.4 \u7ebf\u6027PBG\u7684\u9009\u62e9\u6027\u90e8\u5206\u8131\u4fdd\u62a4<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u5c061.46 g\u7ebf\u6027PBG\uff08\u805a\u5408\u5ea651\uff09\u6eb6\u4e8e15 mL TFA\uff0c\u52a0\u51650.35 mL 33% HBr\/\u4e59\u9178\u6eb6\u6db2\uff0c\u5ba4\u6e29\u6405\u62cc3 h\uff1b\u53cd\u5e94\u6db2\u5012\u5165\u4e59\u919a\u6c89\u6dc0\uff0c\u8fc7\u6ee4\u5f97\u6a59\u8272\u56fa\u4f53\uff1b\u7ecfTHF\/\u5c11\u91cfDMF\u91cd\u6eb6\u3001\u4e59\u919a\u518d\u6c89\u6dc0\u7eaf\u5316\uff0c\u771f\u7a7a\u5e72\u71e5\u5f97\u767d\u8272\u8131\u4fdd\u62a4PBG-COOH\uff0c\u8131\u4fdd\u62a4\u738731 mol%\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.5 G0\uff5eG3\u4ee3\u6811\u679d\u72b6PBG\u7684\u5408\u6210<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>G0\u4ee3\u5408\u6210<\/strong>\uff1a0.141 g PBG-COOH\uff080.220 mmol -COOH\uff09\u4e0e1.10 g\u7ebf\u6027PBG\uff08\u8fc7\u91cf25%\uff09\u6eb6\u4e8e6 mL\u65e0\u6c34DMF\uff1b\u52a0\u51651.72 mL 10% DIC\/DMF\u30010.149 g HOBt\u30010.8 mL\u4e09\u4e59\u80fa\uff0c\u5ba4\u6e29\u53cd\u5e9424 h\uff1b\u52a0\u51650.50 mL\u6b63\u5df1\u80fa\u6dec\u706d\u6d3b\u6027\u916f\uff0c\u7532\u9187\u6c89\u6dc0\u3001\u8fc7\u6ee4\uff1b\u5236\u5907\u7ea7SEC\u7eaf\u5316\uff0c\u771f\u7a7a\u5e72\u71e5\u5f97G0-PBG\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>G1\uff5eG3\u4ee3\u5408\u6210<\/strong>\uff1aG0-PBG\u7ecf0.3 eq HBr\/TFA\u8131\u4fdd\u62a4\uff08\u8131\u4fdd\u62a4\u738732 mol%\uff09\uff0c\u4e0e\u7ebf\u6027PBG\u5728\u65e0\u6c34DMSO\u4e2d\u5076\u805424 h\uff0cSEC\u7eaf\u5316\u5f97G1-PBG\uff1b\u91cd\u590d\u201c\u8131\u4fdd\u62a4\u2013\u63a5\u679d\u201d\u5faa\u73af\uff0c\u4f9d\u6b21\u5408\u6210G2\u3001G3-PBG\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.6 \u7ed3\u6784\u8868\u5f81<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u00b9H NMR\uff1aCDCl\u2083\u6216d\u2086-DMSO\u4e3a\u6eb6\u5242\uff0c\u6d4b\u5b9a\u805a\u5408\u5ea6\u3001\u8131\u4fdd\u62a4\u7387\u4e0e\u6784\u8c61\uff1b\u00b9\u2079F NMR\uff1a\u4e09\u6c1f\u82ef\u7532\u919b\u884d\u751f\u5316\uff0c\u5b9a\u91cf\u7aef\u57fa\u4f2f\u80fa\u542b\u91cf\uff1bSEC-MALLS\uff1aN,N-\u4e8c\u7532\u57fa\u4e59\u9170\u80fa\u4e3a\u6d41\u52a8\u76f8\uff0c\u6d4b\u5b9a\u7edd\u5bf9\u5206\u5b50\u91cf\u4e0e\u5206\u5e03\uff1bDLS\uff1a25\u2103\uff0c90\u00b0\u6563\u5c04\u89d2\uff0c\u6d4b\u5b9a\u6d41\u4f53\u529b\u5b66\u76f4\u5f84\uff1b\u9178\u78b1\u6ef4\u5b9a\uff1a\u9a8c\u8bc1\u7fa7\u57fa\u542b\u91cf\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">3 \u7ed3\u679c\u4e0e\u8ba8\u8bba<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">3.1 Glu-NCA\u5355\u4f53\u5236\u5907<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e09\u5149\u6c14\u4e0eBz-Glu\u5728\u65e0\u6c34\u56de\u6d41\u6761\u4ef6\u4e0b\u53d1\u751f\u73af\u5316\u53cd\u5e94\uff0c\u751f\u6210Glu-NCA\u3002\u00b9H NMR\u663e\u793a\u7279\u5f81\u5cf0\u4e0e\u7ed3\u6784\u5b8c\u5168\u5339\u914d\uff0c\u5355\u4f53\u7eaf\u5ea6&gt;95%\u3002\u5168\u7a0b\u65e0\u6c34\u65e0\u6c27\u53ef\u907f\u514dNCA\u6c34\u89e3\uff0c\u4fdd\u8bc1\u540e\u7eed\u805a\u5408\u53ef\u63a7\u6027\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.2 \u7ebf\u6027PBG\u7684\u4f4e\u6e29\u5f00\u73af\u805a\u5408<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">NCA\u5f00\u73af\u805a\u5408\u4e2d\uff0c\u4f2f\u80fa\u5f15\u53d1\u5242\u8fdb\u653b\u7fb0\u57fa\uff0c\u5f00\u73af\u91ca\u653eCO\u2082\u5e76\u5f62\u6210\u9170\u80fa\u952e\u3002<strong>0\u2103\u4f4e\u6e29<\/strong>\u663e\u8457\u6291\u5236\u94fe\u7aef\u73af\u5316\u526f\u53cd\u5e94\uff1a\u5ba4\u6e29\u805a\u5408\u65f6\u4f2f\u80fa\u7aef\u57fa\u4fdd\u7559\u7387\u4ec570%\uff0c\u800c0\u2103\u805a\u5408\u53ef\u8fbe98%\u3002\u00b9H NMR\u8ba1\u7b97\u805a\u5408\u5ea6\u4e3a26\uff0cSEC\u663e\u793aMw\/Mn=1.10\uff0c\u5206\u5b50\u91cf\u5206\u5e03\u7a84\uff0c\u4e3a\u63a5\u679d\u53cd\u5e94\u63d0\u4f9b\u9ad8\u6d3b\u6027\u5e95\u7269\u3002\u7aef\u57fa\u7a33\u5b9a\u6027\u6d4b\u8bd5\u8868\u660e\uff1a5\u2103\u6c2e\u6c14\u50a8\u5b584\u5468\uff0c\u4f2f\u80fa\u4fdd\u7559\u7387\u7ef4\u630190%\uff0c\u5ba4\u6e29\u50a8\u5b58\u964d\u81f378%\uff0c\u63d0\u793a\u7ebf\u6027PBG\u9700\u4f4e\u6e29\u907f\u5149\u4fdd\u5b58\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.3 \u7ebf\u6027PBG\u7684\u53ef\u63a7\u8131\u4fdd\u62a4<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">HBr\/TFA\u9009\u62e9\u6027\u65ad\u88c2\u03b3-\u82c4\u57fa\u916f\uff0c\u4e3b\u94fe\u9170\u80fa\u952e\u7a33\u5b9a\u3002\u901a\u8fc7\u8c03\u63a7HBr\/\u82c4\u57fa\u916f\u6469\u5c14\u6bd4\uff080.25\uff5e0.30:1\uff09\uff0c\u53ef\u7cbe\u51c6\u63a7\u5236\u8131\u4fdd\u62a4\u7387\u572830%\uff5e40%\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img decoding=\"async\" width=\"1024\" height=\"777\" src=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1024x777.png\" alt=\"\" class=\"wp-image-571\" style=\"aspect-ratio:1.3178957105797655;width:304px;height:auto\" srcset=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1024x777.png 1024w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-300x228.png 300w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-768x583.png 768w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-600x456.png 600w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image.png 1117w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><strong>\u56fe1 \u7ebf\u6027PBG\u8131\u4fdd\u62a4\u524d\u540e\u00b9H NMR\u8c31\u56fe<\/strong><br>\uff08a\uff09\u8131\u4fdd\u62a4\u524d\uff1b\uff08b\uff09\u8131\u4fdd\u62a4\u540e\uff1b\u5cf01\uff1a\u82c4\u57fa\u4e9a\u7532\u57fa\u8d28\u5b50\uff1b\u5cf02\uff1a\u4e3b\u94fe\u6b21\u7532\u57fa\u8d28\u5b50<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u00b9H NMR\u4e2d\uff0c4.9\uff5e5.0 ppm\u82c4\u57fa\u8d28\u5b50\u79ef\u5206\u964d\u4f4e\uff0c3.7\uff5e4.4 ppm\u4e3b\u94fe\u6b21\u7532\u57fa\u79ef\u5206\u4e0d\u53d8\uff0c\u8ba1\u7b97\u5f97\u8131\u4fdd\u62a4\u738731%\uff0c\u4e0e\u6ef4\u5b9a\u7ed3\u679c\u4e00\u81f4\u3002\u8131\u4fdd\u62a4\u524d\u540eSEC\u66f2\u7ebf\u91cd\u5408\uff0cMw\/Mn\u22481.04\uff0c\u65e0\u660e\u663e\u94fe\u964d\u89e3\uff0c\u8131\u4fdd\u62a4\u9009\u62e9\u6027\u4f18\u5f02\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.4 G0\u4ee3\u5408\u6210\u4e0eSEC\u8868\u5f81<\/h3>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img decoding=\"async\" width=\"1024\" height=\"584\" src=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1-1024x584.png\" alt=\"\" class=\"wp-image-572\" style=\"aspect-ratio:1.7534448160535117;width:512px;height:auto\" srcset=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1-1024x584.png 1024w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1-300x171.png 300w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1-768x438.png 768w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1-600x342.png 600w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-1.png 1138w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><strong>\u56fe2 G0\u4ee3\u7c97\u4ea7\u7269SEC\u6d41\u51fa\u66f2\u7ebf<\/strong><br>\uff08\u5de6\u5cf0\uff1aG0\u63a5\u679d\u805a\u5408\u7269\uff1b\u53f3\u5cf0\uff1a\u672a\u53cd\u5e94\u7ebf\u6027PBG\uff09<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">G0\u4ee3\u5728DMF\u4e2d\u5408\u6210\uff0c\u63a5\u679d\u738762%\uff0c\u5076\u8054\u6548\u738741%\uff0cMw\/Mn=1.04\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.5 \u6811\u679d\u72b6PBG\u7684\u9010\u7ea7\u6784\u5efa\u4e0e\u6eb6\u5242\u6548\u5e94<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u9ad8\u4ee3\u5408\u6210\u65f6\uff0c<strong>\u6eb6\u5242\u6548\u5e94\u663e\u8457<\/strong>\uff1aDMF\u4e2dPBG\u5448\u521a\u6027\u03b1-\u87ba\u65cb\uff0c\u7a7a\u95f4\u4f4d\u963b\u5927\uff1bDMSO\u7834\u574f\u5206\u5b50\u5185\u6c22\u952e\uff0cPBG\u8f6c\u4e3a\u65e0\u89c4\u7ebf\u56e2\uff0c\u6d3b\u6027\u4f4d\u70b9\u5145\u5206\u66b4\u9732\uff0c\u663e\u8457\u63d0\u5347\u63a5\u679d\u6548\u7387\u3002G0\uff5eG3\u4ee3\u7ed3\u6784\u53c2\u6570\u5982\u88681\u6240\u793a\uff1aG3\u4ee3\u5206\u5b50\u91cf\u8fbe1.1\u00d710\u2076\uff0cMw\/Mn\u22641.06\uff0c\u5355\u5206\u6563\u6027\u8fdc\u4f18\u4e8e\u201cgrafting from\u201d\u6cd5\u3002\u968f\u4ee3\u6570\u589e\u52a0\uff0c\u652f\u5316\u5ea6\u75316.6\u589e\u81f3165\uff0c\u4f46G2\u2192G3\u652f\u5316\u5ea6\u589e\u5e45\u653e\u7f13\uff08\u4ec52.3\u500d\uff09\uff0c\u5f52\u56e0\u4e8e\u5206\u5b50\u5185\u90e8\u7a7a\u95f4\u62e5\u6324\uff0c\u6d3b\u6027\u4f4d\u70b9\u88ab\u5c4f\u853d\uff0c\u6269\u6563\u53d7\u9650\u6210\u4e3a\u9ad8\u4ee3\u589e\u957f\u7684\u4e3b\u8981\u74f6\u9888\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u88681 G0\uff5eG3\u4ee3\u6811\u679d\u72b6PBG\u7ed3\u6784\u53c2\u6570<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>\u4ee3\u6570<\/th><th>M\u2099\uff08MALLS\uff09<\/th><th>Mw\/Mn<\/th><th>\u63a5\u679d\u7387<\/th><th>\u5076\u8054\u6548\u7387<\/th><th>\u652f\u5316\u5ea6f\u2099<\/th><\/tr><\/thead><tbody><tr><td>G0<\/td><td>5.3\u00d710\u2074<\/td><td>1.04<\/td><td>62%<\/td><td>41%<\/td><td>6.6<\/td><\/tr><tr><td>G1<\/td><td>1.3\u00d710\u2075<\/td><td>1.06<\/td><td>38%<\/td><td>30%<\/td><td>21<\/td><\/tr><tr><td>G2<\/td><td>4.9\u00d710\u2075<\/td><td>1.03<\/td><td>46%<\/td><td>50%<\/td><td>96<\/td><\/tr><tr><td>G3<\/td><td>1.1\u00d710\u2076<\/td><td>1.03<\/td><td>32%<\/td><td>21%<\/td><td>165<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">3.6 \u6eb6\u5242\u8bf1\u5bfc\u6784\u8c61\u8f6c\u53d8<\/h3>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img decoding=\"async\" width=\"857\" height=\"1024\" src=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-857x1024.jpg\" alt=\"\" class=\"wp-image-573\" style=\"aspect-ratio:0.8369115739681087;width:429px;height:auto\" srcset=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-857x1024.jpg 857w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-251x300.jpg 251w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-768x917.jpg 768w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-600x717.jpg 600w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2.jpg 1158w\" sizes=\"(max-width: 857px) 100vw, 857px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><strong>\u56fe4 G0\u4e0eG3\u4ee3PBG\u5728DMF\u4e0eDMSO\u4e2d\u7684\u00b9H NMR\u8c31\u56fe\uff08\u6b21\u7532\u57fa\u533a\u57df\uff09<\/strong><br>\uff08a\u3001c\uff1aDMF\u4e2d\u03b1-\u87ba\u65cb\uff1bb\u3001d\uff1aDMSO\u4e2d\u65e0\u89c4\u7ebf\u56e2\uff09<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u00b9H NMR\u663e\u793a\uff1aDMF\u4e2dPBG\u4e3b\u94fe\u6b21\u7532\u57fa\u5cf0\u5c16\u9510\u9ad8\u573a\uff0c\u5448<strong>\u03b1-\u87ba\u65cb\u6784\u8c61<\/strong>\uff1bDMSO\u4e2d\u5cf0\u5bbd\u5316\u4f4e\u573a\u4f4d\u79fb\uff0c\u8f6c\u4e3a<strong>\u65e0\u89c4\u7ebf\u56e2<\/strong>\u3002\u8be5\u8f6c\u53d8\u5728\u9ad8\u5ea6\u652f\u5316\u7684G3\u4ee3\u4e2d\u4ecd\u53ef\u5b9e\u73b0\uff0c\u8868\u660e\u62d3\u6251\u7ed3\u6784\u4e0d\u5f71\u54cd\u4e3b\u94fe\u6784\u8c61\u53ef\u8c03\u6027\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.7 \u7aef\u57fa\u6d3b\u6027\u9a8c\u8bc1<\/h3>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img decoding=\"async\" width=\"724\" height=\"1024\" src=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-724x1024.png\" alt=\"\" class=\"wp-image-574\" style=\"aspect-ratio:0.7070267795834287;width:372px;height:auto\" srcset=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-724x1024.png 724w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-212x300.png 212w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-768x1087.png 768w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2-600x849.png 600w, https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/image-2.png 778w\" sizes=\"(max-width: 724px) 100vw, 724px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><strong>\u56fe5 \u7ebf\u6027PBG\u7aef\u57fa\u00b9\u2079F NMR\u5206\u6790<\/strong><br>\uff08a\uff09\u53cd\u5e94\u524d\u6d3b\u6027\u7aef\u57fa\uff1b\uff08b\uff09\u53cd\u5e94\u540e\u672a\u53cd\u5e94\u4fa7\u94fe\u7aef\u57fa\uff09<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u00b9\u2079F NMR\u8bc1\u5b9e\u4f4e\u6e29\u5408\u6210\u7684\u7ebf\u6027PBG\u7aef\u57fa\u4f2f\u80fa\u4fdd\u7559\u7387&gt;98%\uff0c\u63a5\u679d\u540e\u672a\u53cd\u5e94\u94fe\u7aef\u6d3b\u6027\u663e\u8457\u4e0b\u964d\uff0c\u7a7a\u95f4\u4f4d\u963b\u662f\u63a5\u679d\u6548\u7387\u53d7\u9650\u7684\u4e3b\u56e0\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.8 \u6d41\u4f53\u529b\u5b66\u5c3a\u5bf8\u5206\u6790<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">DLS\u6d4b\u5b9a\u6d41\u4f53\u529b\u5b66\u76f4\u5f84\uff1aDMF\u4e2dG3\u4ee3\u4e3a24.5 nm\uff08\u87ba\u65cb\u7d27\u51d1\uff09\uff0cDMSO\u4e2d\u4e3a34.5 nm\uff08\u7ebf\u56e2\u8212\u5c55\uff09\uff0c\u7c92\u5f8410\uff5e35 nm\uff0c\u7b26\u5408\u4f53\u5185\u957f\u5faa\u73af\u8981\u6c42\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">4 \u7ed3\u8bba<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u672c\u6587\u901a\u8fc7<strong>\u4f4e\u6e29\u5f00\u73af\u805a\u5408\u3001\u53ef\u63a7\u8131\u4fdd\u62a4\u3001DMSO\u8f85\u52a9\u9010\u6b65\u63a5\u679d<\/strong>\uff0c\u6210\u529f\u5408\u6210G0\uff5eG3\u4ee3\u5355\u5206\u6563\u6811\u679d\u72b6PBG\u3002\u4f4e\u6e29\u805a\u5408\u6291\u5236\u526f\u53cd\u5e94\uff0c\u4fdd\u8bc1\u7aef\u57fa\u9ad8\u6d3b\u6027\uff1b\u53ef\u63a7\u8131\u4fdd\u62a4\u7cbe\u51c6\u5f15\u5165\u63a5\u679d\u4f4d\u70b9\uff1bDMSO\u6d88\u9664\u03b1-\u87ba\u65cb\u4f4d\u963b\uff0c\u5b9e\u73b0\u9ad8\u4ee3\u9ad8\u6548\u63a5\u679d\u3002G3\u4ee3\u5206\u5b50\u91cf\u8fbe1.1\u00d710\u2076\uff0cMw\/Mn\u22641.06\uff0c\u517c\u5177\u9ad8\u5355\u5206\u6563\u6027\u4e0e\u6eb6\u5242\u54cd\u5e94\u6784\u8c61\u8f6c\u53d8\u7279\u6027\u3002\u8be5\u6811\u679d\u72b6\u591a\u80bd\u4e3a\u836f\u7269\u9012\u9001\u3001\u86cb\u767d\u6a21\u62df\u4e0e\u751f\u7269\u533b\u7528\u6750\u6599\u5f00\u53d1\u63d0\u4f9b\u4e86\u91cd\u8981\u6280\u672f\u652f\u6491\uff0c\u5177\u6709\u5e7f\u9614\u5e94\u7528\u524d\u666f\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u53c2\u8003\u6587\u732e<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">[1] Whitton G, Gauthier M. Arborescent Polypeptides from \u03b3-Benzyl L-Glutamic Acid[J]. Journal of Polymer Science Part A: Polymer Chemistry, 2013, 51(24): 5270-5279.<br>[2] Klok H A, Rodriguez-Hernandez J. Dendrigraft Polypeptides from L-Lysine N-Carboxyanhydrides[J]. Macromolecules, 2002, 35(24): 8718-8723.<br>[3] \u738b\u6d69, \u674e\u5a1f. \u6811\u679d\u72b6\u591a\u80bd\u7684\u5408\u6210\u4e0e\u751f\u7269\u533b\u5b66\u5e94\u7528[J]. \u9ad8\u5206\u5b50\u5b66\u62a5, 2020, (5): 489-502.<br>[4] Maeda K, Kamiya N, Yashima E. Conformational Control of Poly(\u03b3-benzyl L-glutamate) in Solution[J]. Chemistry &#8211; A European Journal, 2004, 10(16): 4000-4010.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-3a88641f wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/Arborescent-Polypeptides-from-c-Benzyl-L-Glutamic-Acid-1.pdf\" type=\"application\/pdf\" style=\"width:100%;height:200px\" aria-label=\"Embed of Arborescent Polypeptides from c-Benzyl L-Glutamic Acid.\"><\/object><a id=\"wp-block-file--media-fd7af48e-21e4-4af0-a8a9-af3bdeb45cb8\" href=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/Arborescent-Polypeptides-from-c-Benzyl-L-Glutamic-Acid-1.pdf\">Arborescent Polypeptides from c-Benzyl L-Glutamic Acid<\/a><a href=\"https:\/\/www.shiweitec.com\/wp-content\/uploads\/2026\/05\/Arborescent-Polypeptides-from-c-Benzyl-L-Glutamic-Acid-1.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-fd7af48e-21e4-4af0-a8a9-af3bdeb45cb8\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6458\u8981 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\u805a\u8c37\u6c28\u9178\uff08PGA\uff09\u662f\u4e00\u7c7b\u7531\u8c37\u6c28\u9178\u5355\u4f53\u901a\u8fc7\u9170\u80fa\u952e\u8fde\u63a5\u5f62\u6210\u7684\u751f\u7269\u53ef\u964d\u89e3\u3001\u4f4e\u514d\u75ab\u539f\u6027\u591a\u80bd\uff0c\u5728\u836f\u7269\u8f7d\u4f53\u3001\u7ec4\u7ec7\u5de5\u7a0b\u3001\u7eb3\u7c73\u9012\u9001\u7cfb\u7edf\u7b49\u9886\u57df\u5177\u6709\u91cd\u8981\u5e94\u7528\u4ef7\u503c\u3002\u6839\u636e\u8fde\u63a5\u65b9\u5f0f\uff0cPGA\u5206\u4e3a\u03b1-\u805a\u8c37\u6c28\u9178\u4e0e\u03b3-\u805a\u8c37\u6c28\u9178\uff1a\u03b3-PGA\u591a\u7531\u5fae\u751f\u7269\u53d1\u9175\u5236\u5907\uff0c\u800c\u03b1-PGA\u53ca\u62d3\u6251\u7ed3\u6784\u53ef\u63a7\u7684\u884d\u751f\u7269\uff08\u68b3\u5f62\u3001\u661f\u5f62\u3001\u6811\u679d\u72b6\uff09\u9700\u901a\u8fc7\u5316\u5b66\u5408\u6210\u5b9e\u73b0\u3002 \u6811\u679d\u72b6\u591a\u80bd\uff08Arborescent 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mmol\uff09Glu-NCA\u6eb6\u4e8e20 mL\u65e0\u6c34DMF\uff0c0\u2103\u6405\u62cc\u6eb6\u89e3\uff1b\u52a0\u5165200 \u03bcL\uff081.52 mmol\uff09\u6b63\u5df1\u80fa\uff08\u5355\u4f53\/\u5f15\u53d1\u5242=20\uff09\uff0c0\u2103\u5bc6\u5c01\u53cd\u5e9472 h\uff1b\u53cd\u5e94\u6db2\u5012\u5165\u7532\u9187\u6c89\u6dc0\uff0c\u8fc7\u6ee4\u3001\u7532\u9187\u591a\u6b21\u6d17\u6da4\uff1b\u771f\u7a7a\u5e72\u71e5\u5f97\u767d\u8272\u7c89\u672b\u7ebf\u6027PBG\uff0c\u4ea7\u738780%\u3002 2.4 \u7ebf\u6027PBG\u7684\u9009\u62e9\u6027\u90e8\u5206\u8131\u4fdd\u62a4 \u5c061.46 g\u7ebf\u6027PBG\uff08\u805a\u5408\u5ea651\uff09\u6eb6\u4e8e15 mL TFA\uff0c\u52a0\u51650.35 mL 33% HBr\/\u4e59\u9178\u6eb6\u6db2\uff0c\u5ba4\u6e29\u6405\u62cc3 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onto\uff09\u201d\u7b56\u7565\uff0c\u4ee5Glu-NCA\u4e3a\u5355\u4f53\uff0c\u7ecf\u4f4e\u6e29\u5f00\u73af\u805a\u5408\u3001\u53ef\u63a7\u8131\u4fdd\u62a4\u3001\u9010\u6b65\u63a5\u679d\uff0c\u5408\u6210G0\uff5eG3\u4ee3\u6811\u679d\u72b6PBG\u3002\u7cfb\u7edf\u7814\u7a76\u805a\u5408\u6e29\u5ea6\u3001\u8131\u4fdd\u62a4\u5ea6\u3001\u6eb6\u5242\u6548\u5e94\u4e0e\u5076\u8054\u6761\u4ef6\u5bf9\u4ea7\u7269\u7ed3\u6784\u4e0e\u6027\u80fd\u7684\u5f71\u54cd\uff0c\u9610\u660e\u6eb6\u5242\u8bf1\u5bfc\u6784\u8c61\u8f6c\u53d8\u673a\u5236\uff0c\u4e3a\u9ad8\u6027\u80fd\u751f\u7269\u533b\u7528\u591a\u80bd\u6750\u6599\u5f00\u53d1\u63d0\u4f9b\u65b0\u8def\u5f84\u3002 2 \u5b9e\u9a8c\u90e8\u5206 2.1 \u8bd5\u5242\u4e0e\u4eea\u5668 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\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178N-\u7fa7\u57fa\u9178\u9150\uff08Glu-NCA\uff09\u7684\u5408\u6210 \u5c0610.0 g\uff0842.0 mmol\uff09Bz-Glu\u60ac\u6d6e\u4e8e300 mL\u65e0\u6c34\u4e59\u9178\u4e59\u916f\uff0c\u6c2e\u6c14\u4fdd\u62a4\u4e0b\u52a0\u70ed\u81f3\u56de\u6d41\uff1b\u7f13\u6162\u52a0\u51654.8 g\uff0816 mmol\uff09\u4e09\u5149\u6c14\uff0c\u56de\u6d413 h\uff1b\u51b0\u6c34\u6d74\u51b7\u53741 h\uff0c\u4f9d\u6b21\u7528\u51b0\u6c34\u30010.5% NaHCO\u2083\u6c34\u6eb6\u6db2\u5feb\u901f\u6d17\u6da4\uff1b\u6709\u673a\u76f8\u7ecf\u65e0\u6c34MgSO\u2084\u5e72\u71e5\u3001\u8fc7\u6ee4\u3001\u51cf\u538b\u6d53\u7f29\u81f3100 mL\uff1b\u52a0\u5165\u7b49\u4f53\u79ef\u51b7\u6b63\u5df1\u70f7\uff0c-20\u2103\u7ed3\u6676\u8fc7\u591c\uff1b\u6c2e\u6c14\u4fdd\u62a4\u4e0b\u8fc7\u6ee4\uff0c\u771f\u7a7a\u5e72\u71e5\u5f97\u767d\u8272\u6676\u4f53Glu-NCA\uff0c\u4ea7\u738792%\u3002 2.3 \u7ebf\u6027\u805a\u03b3-\u82c4\u57fa-L-\u8c37\u6c28\u9178\uff08PBG\uff09\u7684\u5f00\u73af\u805a\u5408 \u5c068.00 g\uff0830.4 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